Exocyst Requirement for Endocytic Traffic Directed Toward ...- guerriero insetticida sds ,Aug 08, 2007·The immunoprecipitates were resolved by SDS-PAGE and Western blots were sequentially probed with antibodies that recognize Rab11a or Sec15A. To confirm that Sec15CT can interact with Rab11a in vivo, we generated stable cell lines expressing GFP-Sec15CT or GFP-Sec15CT(NA), which were subsequently infected with GFP/HA-Rab11aSV adenovirus.Molecular investigation of Tuscan sweet cherries sampled ...Jan 01, 2021·Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We …



Exocyst Requirement for Endocytic Traffic Directed Toward ...

Aug 08, 2007·The immunoprecipitates were resolved by SDS-PAGE and Western blots were sequentially probed with antibodies that recognize Rab11a or Sec15A. To confirm that Sec15CT can interact with Rab11a in vivo, we generated stable cell lines expressing GFP-Sec15CT or GFP-Sec15CT(NA), which were subsequently infected with GFP/HA-Rab11aSV adenovirus.

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A novel tripartite motif involved in aquaporin topogenesis ...

Jul 15, 2007·Tagged AQP1 was readily distinguished from wild-type protein by its slower migration on SDS-PAGE (Fig. 6a, lanes 1–4), and only tagged protein was reactive to …

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A model of dirigent proteins derived from structural and ...

Mar 24, 2012·Introduction. Dirigent proteins (DIRs) are critically involved in the formation of lignans, a diverse and widely distributed class of secondary plant metabolites exhibiting interesting pharmacological activities and implicated in natural plant defense [[1, 2]].The biosynthesis of lignans is typically initiated by the one‐electron oxidation of two monolignols (phenylpropanoids) and their ...

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SECTION 1: Identification

G40, Supreme Shine Protectant (21-115A): G4016 01/03/17 Page 2 of 8 ,, , G4024SP Pictograms

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Medical Management Guidelines for Acute Chemical Exposure

Jan 27, 2016·Introduction The Guidelines contains 27 chemical-specific medical management protocols for the treatment of exposed patients. The chemicals were selected using the following criteria: (1) prevalence at hazardous waste sites, (2) ability to cause serious adverse health effects, and (3) frequency of reported deaths, injuries, or transportation accidents.

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US6903202B2 - DNA encoding proteins that inhibit Hsp70 ...

US6903202B2 US10/093,045 US9304502A US6903202B2 US 6903202 B2 US6903202 B2 US 6903202B2 US 9304502 A US9304502 A US 9304502A US …

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Expression and purification of the extracellular domain of ...

Dec 01, 2020·Mutations in RET lead to a variety of human diseases. For example, gain-of-function mutations are strongly associated with cancers, including non-small-cell cancer (NSCC) and MEN 2A, while loss-of-function mutations contribute to Parkinson's disease and Hirschsprung's disease [, , ].In the case of MEN 2A, point mutations of the cysteine residues in the CRD, most commonly C634R, lead to …

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Microplastic in wild populations of the omnivorous crab ...

Aug 01, 2019·Ten mL of the 25% anionic detergent sodium dodecyl sulphate (250 g SDS L −1) (Sigma-Aldrich®) was added to each gastrointestinal tract sample immediately after dissection and samples were left to incubate at 50 °C for 24 h. Subsequently, 5 mL of Enzyme F …

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Ligand-triggered allosteric ADP release primes a plant NLR ...

After washing, the Ni beads were analyzed by SDS-PAGE and Coomassie brilliant blue staining. To assay the effect of PBL2 or PBL2 UMP on the ADP-binding activity of ZAR-RKS1, the [2,8-3 H]-ADP-ZAR1-RKS1 complex protein bound to Ni resins was incubated with different concentrations of PBL2 or PBL2 UMP, and then each sample was pelleted by ...

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Tight Junction–associated MARVEL Proteins MarvelD3 ...

Feb 17, 2010·INTRODUCTION. The tight junction is a network of transmembrane and peripheral proteins that form a semipermeable barrier to paracellular flux (Farquhar and Palade, 1963; Claude and Goodenough, 1973).The functions of some of these proteins have been defined in recent years, including roles for the peripheral membrane proteins ZO-1 and -2 in recruiting transmembrane claudin proteins …

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Molecular investigation of Tuscan sweet cherries sampled ...

Jan 01, 2021·Sweet cherry (Prunus avium L.) is a stone fruit widely consumed and appreciated for its organoleptic properties, as well as its nutraceutical potential. We …

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Recombinant Human/Mouse/Rat Activin A Protein (338-AC ...

Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 is 0.2‑1.2 ng/mL.

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Tight Junction–associated MARVEL Proteins MarvelD3 ...

Feb 17, 2010·INTRODUCTION. The tight junction is a network of transmembrane and peripheral proteins that form a semipermeable barrier to paracellular flux (Farquhar and Palade, 1963; Claude and Goodenough, 1973).The functions of some of these proteins have been defined in recent years, including roles for the peripheral membrane proteins ZO-1 and -2 in recruiting transmembrane claudin proteins …

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Functional Fluorescent Protein Insertions in Herpes ...

Mutagenesis studies have been used to identify functionally important regions of gB, which are found throughout the ectodomain. Random linker-insertion mutagenesis, using linkers of five amino acids, revealed functional insertion constructs within the N-terminus, and within a loop in FR2 .A separate study utilizing insertions of just two alanines described more sites that tolerated the smaller ...

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Sodium dodecyl sulphate-coated alumina for the extraction ...

May 31, 2006·The results obtained are depicted in Fig. 1.In the absence of SDS, BFs were not retained on γ-alumina. The retention of BFs on hemimicelles, which have a hydrophobic surface, was clearly dependent on analyte polarity (e.g. log K o/w were 1.5, 2.5 and 2.7 for CB, TB and FB, respectively) and it increased as the amount of hemimicelles did. The formation of minute amounts of admicelles was ...

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Binding of the Magnaporthe oryzae Chitinase MoChia1 by a ...

To defend against pathogens, plants have developed complex immune systems, including plasma membrane receptors that recognize pathogen-associated molecular patterns, such as chitin from fungal cell walls, and mount a defense response. Here, we identify a chitinase, MoChia1 ( Magnaporthe oryzae chitinase 1), secreted by M. oryzae , a fungal pathogen of rice ( Oryza sativa ).

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(PDF) Synthesis and assembly of functionally active human ...

zyxwvutsrqp zyxwvuts Eur. J. Biochem. 211, 19-26 (1993) 0FEBS 1993 zyxw Synthesis and assembly of functionally active human vascular endothelial growth factor homodimers in insect cells Bernd L. FIEBICH, Birgit JAGER, Claudia SCHOLLMANN, Karin WEINDEL, Jorg WILTING ', Georg KOCHS, Dieter MARME, Hubert HUG and Herbert A. WEICH Institute of Molecular Cell Biology, University of …

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Diminished MTORC1-Dependent JNK Activation Underlies the ...

Wong et al. identify an MTORC1-JNK signaling axis in Drosophila and mouse neurons required for synaptic development, which is attenuated upon endolysosomal dysfunction. The authors’ findings suggest a possible therapeutic strategy that may be beneficial for tackling the neurodevelopmental defects commonly observed in lysosomal storage diseases with CNS involvement.

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Post-translational regulation and trafficking of the ...

The 40 and 30 kDa signals are absent in SDS-treated extracts of rd21-1 mutant plants (Figure 7D, lane 7), indicating that these signals are derived from RD21, and represent iRD21 and mRD21, respectively. The increase in DCG-04 labeling upon SDS treatment is correlated with a destabilisation of rubisco in Arabidopsis leaf extracts.

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Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated ...

Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid, 23 kDa member of the glutathione peroxidase antioxidant enzyme family.

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(PDF) Exocyst Requirement for Endocytic Traffic Directed ...

The immunoprecipitates were resolved by SDS-PAGE and Western blots were sequentially probed with antibodies that recognize Rab11a or Sec15A. ATP-dependent values were normalized to control reactions with 125I-IgA from the apical domain for 10 min, the cells that contained an ATP-regenerating system and cytosol, but were washed and chased in the ...

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Subject: "1,3-beta-glucan synthase" - PubAg Search Results

You searched for: Subject "1,3-beta-glucan synthase" Remove constraint Subject: "1,3-beta-glucan synthase"

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Nutrients | Free Full-Text | The Edible Insect Gryllus ...

Accumulation of reactive oxygen species (ROS) in response to excess alcohol exposure is a major cause of gut barrier disruption and lipopolysaccharide (LPS)-induced hepatic inflammation, as well as liver steatosis and apoptosis. This study was designed to investigate protective effects of the cricket Gryllus bimaculatus, an edible insect recognized by the Korea Food and Drug Administration ...

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HDAC4 Controls Muscle Homeostasis through Deacetylation of ...

Luo et al. use class IIa HDAC inhibitors, along with skeletal-muscle-specific and whole-body inducible HDAC4 knockout mice, to demonstrate HDAC4 deacetylates three previously undescribed substrates: myosin heavy chain, PGC-1α, and Hsc70. Through these substrates, HDAC4 inhibition leads to rescue of muscle atrophy and increased succinate dehydrogenase activity.

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